Voltage Clamp Neuron. Voltage clamp is one if not the most powerful approach to the study of the voltage gated na channel. In nonspherical cells such as neurons the membrane potential is not clamped distal to the voltage clamp electrode.
This will simulate hh neuron model for applied voltage. The voltage clamp experiment initial set up. The voltage clamp technique is applicable only to spherical cells.
The voltage clamp is an experimental method used by electrophysiologists to measure the ion currents through the membranes of excitable cells such as neurons while holding the membrane voltage at a set level.
Total ionic current and individual components of ionic current is estimating and total ionic current plotting in the top graph as red trace in response to the voltage applied as voltage which is plotted as blue trace in the same graph see fig. In nonspherical cells such as neurons the membrane potential is not clamped distal to the voltage clamp electrode. 1 nacl 0 4 cscl 4 0 3 atp gtp 10 phosphocreatine and 1 qx 314 recording epscs at 70 mv in voltage clamp ipscs at 0 mv. Therefore the spine epsp and the recovered current are linearly related figure 3a.
