Voltage Clamp Cell. To maintain this command voltage level an amplifier must inject current. The voltage clamp is a technique used to control the voltage across the membrane of a small or isopotential area of a nerve cell by an electronic feedback circuit.
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Voltage clamp amplifier in an experiment using the voltage clamp method the investigator controls the membrane voltage in a cell and measures the transmembrane current required to maintain that voltage. In cell attached voltage clamp mode the capacitor provides a low impedance pathway through the patch for fast events such as action potentials resulting in the measurement of a current with an approximately 10 fold larger magnitude than would be measured at steady state for the same magnitude change in membrane potential. The voltage clamp is a technique used to control the voltage across the membrane of a small or isopotential area of a nerve cell by an electronic feedback circuit.
This voltage control is called a command voltage.
Clamping the cell at 0 mv is above threshold which triggers the voltage gated sodium channels to open. Abstract this unit describes the use of whole cell voltage clamping to study voltage gated channels. In cell attached voltage clamp mode the capacitor provides a low impedance pathway through the patch for fast events such as action potentials resulting in the measurement of a current with an approximately 10 fold larger magnitude than would be measured at steady state for the same magnitude change in membrane potential. Voltage clamp amplifier in an experiment using the voltage clamp method the investigator controls the membrane voltage in a cell and measures the transmembrane current required to maintain that voltage.
